First 1 in each heat and next 6 fastest progress to the final.
| Place | Centre | Result | ||
|---|---|---|---|---|
| 1 | Northern Districts | 56.30 | Q | |
| 2 | Western Athletics | 56.78 | q | |
| 3 | Coastal Districts | 57.17 | q | |
| 4 | Southern Hills | 1:03.22 | q | |
| 5 | Reynella East | 1:06.40 | ||
| 6 | Golden Grove B | 1:07.00 |
| Place | Centre | Result | ||
|---|---|---|---|---|
| 1 | Golden Grove | 55.56 | Q | |
| 2 | Mid Coast | 58.56 | q | |
| 3 | Lyndoch & Districts | 1:00.07 | q | |
| 4 | Eastern Districts | 1:00.27 | q | |
| 5 | Hills | 1:07.94 | ||
| 6 | Noarlunga | 1:08.20 |