First 2 in each heat and next 2 fastest progress to the final.
| Place | Centre | Result | ||
|---|---|---|---|---|
| 1 | Adelaide Harriers A | 57.34 | Q | |
| 2 | Plympton A | 59.03 | Q | |
| 3 | Tea Tree Gully A | 59.80 | q | |
| 4 | Western Athletics B | 1:01.72 | ||
| 5 | Port Adelaide B | 1:03.26 | ||
| 6 | GGR/HIL/NYP A | 1:03.72 | ||
| South Coast: Victor Harbor A | DNS |
| Place | Centre | Result | ||
|---|---|---|---|---|
| 1 | Barossa Valley A | 59.84 | Q | |
| 2 | Hallett Cove A | 1:02.23 | Q | |
| 3 | Southern Hills A | 1:03.39 | ||
| 4 | Ingle Farm/Noarlunga A | 1:03.63 | ||
| 5 | Munno Para A | 1:07.69 | ||
| Woodville A | DNS |
| Place | Centre | Result | ||
|---|---|---|---|---|
| 1 | Port Adelaide A | 57.01 | Q | |
| 2 | Enfield A | 1:00.78 | Q | |
| 3 | Western Athletics A | 1:01.05 | q | |
| 4 | Salisbury A | 1:04.79 | ||
| 5 | Eastern Districts A | 1:04.84 | ||
| 6 | Plympton B | 1:06.56 |
| Place | Centre | Result | ||
|---|---|---|---|---|
| 1 | Port Adelaide A | 57.01 | Q | |
| 2 | Adelaide Harriers A | 57.34 | Q | |
| 3 | Barossa Valley A | 59.84 | Q | |
| 4 | Plympton A | 59.03 | Q | |
| 5 | Enfield A | 1:00.78 | Q | |
| 6 | Hallett Cove A | 1:02.23 | Q | |
| 7 | Tea Tree Gully A | 59.80 | q | |
| 8 | Western Athletics A | 1:01.05 | q | |
| 9 | Western Athletics B | 1:01.72 | ||
| 10 | Port Adelaide B | 1:03.26 | ||
| 11 | Southern Hills A | 1:03.39 | ||
| 12 | Ingle Farm/Noarlunga A | 1:03.63 | ||
| 13 | GGR/HIL/NYP A | 1:03.72 | ||
| 14 | Salisbury A | 1:04.79 | ||
| 15 | Eastern Districts A | 1:04.84 | ||
| 16 | Plympton B | 1:06.56 | ||
| 17 | Munno Para A | 1:07.69 | ||
| South Coast: Victor Harbor A | DNS | |||
| Woodville A | DNS |